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Hypothermic modulation of doxorubicin, cisplatin and radiation cytotoxicity in vitro
L Lundgren-Eriksson1, R Hultborn, R Henriksson
1Department of Radiation Sciences, Oncology, Umeå University, Sweden. lotta.lundgren.eriksson@onkologi.umu.se
Background:
The influence of hypothermia on doxorubicin, cisplatin and radiation cytotoxicity was investigated in vitro.
Materials And Methods:
A human glioma cell line (251MG) in early exponential growth was exposed to doxorubicin or cisplatin at various concentrations for 4 hours, or X-irradiation at 28 degrees C or 37 degrees C. The cells continued growing in multi-well plates at 37 degrees C and were counted every third day until the end of the logarithmic phase, on day 13.
Results:
Exposure to doxorubicin 0.05-0.5 microg/ml or cisplatin 1-10 microg/ml caused a dose-dependent inhibition of cell growth with a significantly reduced toxicity when exposed at 28 degrees C as compared to 37 degrees C. Irradiation with 4 Gy also resulted in less toxicity during hypothermia. Chlorpromazine 0.01-10 microg/ml, used to induce hypothermia in vivo (1), neither influenced, cellular growth itself nor interacted with doxorubicin, cisplatin or irradiation.
Conclusion:
Moderate hypothermia (28 degrees C) appears to protect against the cellular insult of doxorubicin, cisplatin and ionising irradiation and their consequences.
