Novel DNA sensor for guanine content
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Updated: Oct 2, 2026

A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
André Del Guerzo1, Andrée Kirsch-De Mesmaeker
1Chimie Organique Physique, Université Libre de Bruxelles, CP 160/08, 50 Avenue F. D. Roosevelt, 1050 Bruxelles, Belgium.
Abstract:
The bifunctional complex [Ru(TAP)(2)POQ-Nmet](2+), 1, formed with a [Ru(TAP)(2)Phen](2+) metallic unit linked to a quinoline moiety, and [Ru(TAP)(2)Phen](2+), 2, as reference, have been tested as photoprobes of DNA. Interestingly, 1 exhibits an emission enhancement of a factor of 16-17 upon binding to calf thymus DNA. Moreover, this emission is modulated by the nucleic base content of the polynucleotide. It varies by almost an order of magnitude from a polynucleotide containing 100% of G-C to a guanine-free nucleic acid where the excited-state lifetime reaches about 2 micros. The origins of these interesting properties are analyzed by comparing 1 with reference 2 in the presence of different polynucleotides.
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