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Identification of Fusarium graminearum in cereal samples by DNA Detection Test Strips
S Knoll1, R F Vogel, L Niessen
1Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Weihenstephaner Steig 16, D-85350 Freising, Weihenstephan, Germany.
Aims:
Development of a fast, sensitive and easy to handle method for the detection of Fusarium graminearum contamination in cereal samples by PCR.
Methods And Results:
DNA Detection Test Strips were used for PCR-product detection and the method was compared to agarose gel electrophoresis. A minimum of 0.26 ng of purified target DNA was detectable with the Test Strip Detection limit in less contaminated samples was slightly lower when gel electrophoresis was used for amplicon detection. In highly contaminated samples, detection limits of both methods were similar.
Conclusions:
Detection of PCR products was performed in 20 min without the need of special technical equipment or hazardous fluorescent DNA dyes.
Significance And Impact Of The Study:
The new method described is useful for the screening of cereals in industrial quality control.