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Determination of piroxicam by solid-phase spectrophotometry in a continuous flow system
María Isabel Pascual-Reguera1, María Jose Ayora-Cañada, María Soledad Castro Ruiz
1Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, E-23071 Jaén, Spain. ipascual@ujaen.es
Summary
A new flow injection UV spectrophotometric method effectively determines piroxicam using Sephadex DEAE-A-25 anion-exchange gel beads for concentration. This technique offers a sensitive and accurate approach for pharmaceutical analysis.
Area of Science:
- Analytical Chemistry
- Spectrophotometry
- Chromatography
Background:
- Accurate determination of pharmaceutical compounds like piroxicam is crucial for quality control.
- Existing methods may lack sensitivity, speed, or simplicity for routine analysis.
- Development of novel analytical techniques can improve drug quantification in pharmaceutical formulations.
Purpose of the Study:
- To develop a simple and efficient flow injection UV spectrophotometric method for piroxicam determination.
- To utilize Sephadex DEAE-A-25 anion-exchange gel for transient retention and concentration of piroxicam.
- To validate the method for its application in analyzing pharmaceutical samples.
Main Methods:
- A monochannel flow injection analysis (FIA) manifold was employed.
- Piroxicam was retained and concentrated on Sephadex DEAE-A-25 packed in a flow cell.
- UV spectrophotometry at 354 nm was used for continuous monitoring on the solid phase.
Main Results:
- The method demonstrated linearity in the concentration range of 0.5-10 microg/ml.
- A low detection limit of 0.1 microg/ml and a relative standard deviation of 1.8% were achieved.
- A sampling rate of 13 samples per hour and accuracy better than 5% were obtained.
Conclusions:
- The developed flow injection UV spectrophotometric method is simple, sensitive, and accurate for piroxicam determination.
- The use of Sephadex DEAE-A-25 as an anion-exchange sorbent provides effective concentration.
- The method is suitable for the routine analysis of piroxicam in pharmaceutical samples.