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Related Experiment Videos

High-throughput screening for biocatalysts.

D Wahler1, J L Reymond

  • 1Departement für Chemie und Biochemie, Universität Bern, Freiestrasse 3, 3012 Bern, Switzerland.

Current Opinion in Biotechnology
|February 19, 2002
PubMed
Summary
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High-throughput enzyme assays have advanced with new substrate types and indicator methods. Recent developments also include Horeau

Area of Science:

  • Biochemistry
  • Enzymology
  • Assay Development

Background:

  • High-throughput screening (HTS) is crucial for enzyme discovery and characterization.
  • Novel assay methodologies are needed to enhance efficiency and scope in enzyme research.

Purpose of the Study:

  • To review recent advancements in high-throughput enzyme assay technologies.
  • To highlight new substrate types and indicator methods for enzyme analysis.

Main Methods:

  • Review of recent literature on fluorogenic, chromogenic, and FRET substrates.
  • Analysis of pH and pM indicator methods in high-throughput enzyme assays.
  • Examination of Horeau's pseudo-enantiomer derivatization for enantioselectivity screening.

Main Results:

Related Experiment Videos

  • Introduction of diverse substrate types, including fluorogenic, chromogenic, and FRET.
  • Successful application of pH and pM indicator methods for enzyme activity monitoring.
  • Adaptation of Horeau's method for high-throughput enantioselectivity assessment.

Conclusions:

  • Significant progress has been made in developing versatile high-throughput enzyme assays.
  • These advancements facilitate efficient enzyme screening and characterization.