Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Directed enzyme evolution.

E T Farinas1, T Bulter, F H Arnold

  • 1Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology, Pasadena, CA 91125, USA.

Current Opinion in Biotechnology
|February 19, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutations in adenine-binding pockets enhance catalytic properties of NAD(P)H-dependent enzymes.

Protein engineering, design & selection : PEDS·2015
Same author

Uncovering rare NADH-preferring ketol-acid reductoisomerases.

Metabolic engineering·2014
Same author

How proteins adapt: lessons from directed evolution.

Cold Spring Harbor symposia on quantitative biology·2010
Same author

Chemoenzymatic synthesis of biotinylated nucleotide sugars as substrates for glycosyltransferases.

Chembiochem : a European journal of chemical biology·2002
Same author

Computationally focusing the directed evolution of proteins.

Journal of cellular biochemistry. Supplement·2002
Same author

Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution.

Protein engineering·2001
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Laboratory evolution generates improved enzymes for industry and research. New biocatalysts aid carbon-carbon bond formation and plant fatty acid production, advancing biological design.

Area of Science:

  • Biotechnology and Synthetic Biology
  • Enzyme Engineering
  • Molecular Evolution

Background:

  • Laboratory evolution is a powerful tool for optimizing enzymes.
  • Enzyme engineering drives innovation in industrial and research applications.
  • Understanding enzyme adaptation is crucial for biological design.

Purpose of the Study:

  • To highlight recent advancements in laboratory evolution for enzyme generation.
  • To showcase novel biocatalysts for specific chemical transformations.
  • To explore the elucidation of enzyme adaptation mechanisms.

Main Methods:

  • Directed evolution techniques for enzyme optimization.
  • Laboratory evolution experiments to test adaptation scenarios.
  • Biocatalyst development for carbon-carbon bond formation and fatty acid production.

Related Experiment Videos

Main Results:

  • Development of enhanced biocatalysts for enantioselective reactions.
  • Successful engineering of enzymes for increased fatty acid production in plants.
  • Progress in understanding the mechanisms underlying enzyme adaptation.

Conclusions:

  • Laboratory evolution continues to yield superior enzymes for diverse applications.
  • Innovations in evolutionary methods expand the possibilities of biological design.
  • Laboratory studies are providing insights into natural enzyme evolution.