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Cytokine gene polymorphisms in acute pancreatitis
K Sargen1, A G Demaine, A N Kingsnorth
1Department of Surgery, Plymouth Postgraduate Medical School, Derriford Hospital. Plymouth, United Kingdom.
JOP : Journal of the Pancreas
|February 20, 2002
Summary
Genetic variations in tumor necrosis factor (TNF) and interleukin-10 (IL-10) genes do not influence acute pancreatitis severity or susceptibility. This study found no significant allelic frequency differences in these cytokine gene loci.
Area of Science:
- Genetics
- Immunology
- Gastroenterology
Background:
- Pro-inflammatory and regulatory cytokines are crucial in acute pancreatitis pathogenesis.
- Genetic loci encoding cytokines exhibit polymorphisms, potentially affecting protein expression.
Purpose of the Study:
- To determine if tumor necrosis factor (TNF) and interleukin-10 (IL-10) gene loci are associated with acute pancreatitis occurrence or severity.
Main Methods:
- Typing of three TNF microsatellite loci (TNFa, TNFb, TNFc), TNF-308 polymorphism, IL-10.G microsatellite locus, and 3 IL-10 5' region polymorphisms using PCR.
- Analysis of 135 acute pancreatitis patients and 107 ethnically matched controls.
- Grouping patients by disease severity using organ failure scores or Atlanta system classification.
Main Results:
- No significant differences in allelic frequencies of studied cytokine gene loci were observed between patients and controls.
- Allelic frequencies did not differ significantly when stratified by disease aetiology or severity.
Conclusions:
- The studied cytokine gene polymorphisms (TNF and IL-10) do not appear to play a role in determining acute pancreatitis severity or susceptibility.