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Related Experiment Videos

Shock wave-mediated molecular delivery into cells.

Tetsuya Kodama1, Apostolos G Doukas, Michael R Hamblin

  • 1BAR314B, Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA. kodama@helix.mgh.harvard.edu

Biochimica Et Biophysica Acta
|February 21, 2002
PubMed
Summary

Shock waves effectively deliver large molecules into cancer cells without harm. This non-toxic method shows promise for applications like gene therapy.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Biophysics

Background:

  • Efficient intracellular delivery of macromolecules is crucial for various biological applications.
  • Current methods for cell permeabilization often involve cytotoxicity.
  • Developing non-invasive and effective delivery techniques is a significant challenge.

Purpose of the Study:

  • To investigate the efficacy of shock wave technology for macromolecule delivery into cancer cells.
  • To determine the impact of molecular weight and shock wave parameters on delivery efficiency.
  • To assess the cytotoxicity associated with shock wave-mediated delivery.

Main Methods:

  • Utilizing a shock tube to generate shock waves for cell permeabilization.
  • Employing fluorescein isothiocyanate-dextran of varying molecular weights to assess delivery.

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  • Quantifying cellular permeabilization and fluorescence intensity via flow cytometry.
  • Evaluating cellular viability using a tetrazolium assay and comparing with digitonin permeabilization.

    • Main Results:

    • Shock waves effectively delivered macromolecules, including those up to 2,000,000 molecular weight, into the cytoplasm.
    • Delivery efficiency and total fluorescence were measurable and dependent on shock wave parameters.
    • The shock wave method demonstrated no significant cytotoxicity to the cancer cell line.
    • Comparison with digitonin showed comparable or superior delivery without toxicity.

    Conclusions:

    • Shock wave technology provides a non-toxic and effective method for intracellular delivery of large molecules.
    • This technique holds potential for advancing applications in gene therapy and drug delivery.
    • Further research into optimizing shock wave parameters could enhance delivery efficiency for therapeutic purposes.