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A cationic sub-micron emulsion (MF59/DOTAP) is an effective delivery system for DNA vaccines
Gary Ott1, Manmohan Singh, Jina Kazzaz
1Chiron Corporation, Mail Stop 4.3, 4560 Horton Street, Emeryville, CA 94608, USA.
Abstract:
A novel cationic emulsion was developed to adsorb plasmid DNA and improve intracellular delivery of plasmid DNA upon immunization. The emulsion developed, had a highly uniform particle and charge distribution. Based on observations with cationic microparticles, the cationic emulsion was evaluated in vivo in mice and rabbits with a model HIV-1 pCMVp55 gag DNA. In both these species, the cationic emulsion engendered higher antibody responses than those obtained with naked DNA. The cationic emulsion also maintained the cellular responses seen with naked DNA at the same doses.