Heterogeneous cell renewal of pancreas in mice: [(3)H]-thymidine autoradiographic investigation
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Published on: December 7, 2010
Yasushi Magami1, Takeshi Azuma, Hideto Inokuchi
1Fourth Department of Internal Medicine, Tokyo Medical University, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan. magami@tokyo-med.ac.jp
Introduction:
Although cell kinetics of the gastrointestinal mucosa has been extensively examined, that of the pancreas has not been fully analyzed.
Aim:
To determine the renewal rate of pancreatic cells directly.
Methodology:
Postnatal proliferative activity and cellular renewal of the parenchymal cells in ICR mouse pancreas were studied by immunohistochemistry and [(3)H]-thymidine autoradiography.
Results:
In the single labeling experiments, the proliferative activity of the parenchymal cells in pancreas showed peaks at a few days after birth, decreased thereafter, and reached a low level at 2 months after birth. Continuous labeling experiments revealed that, in the adult pancreas, the half lives of acinar cells, islet cells, and duct epithelial cells were approximately 70 days, 47 days, and 40 days, respectively. Moreover, in the exocrine pancreas, acinar cells of the peri-insular region proliferated more actively than those of the tele-insular region. The renewal rate of glucagon cells was more rapid than that of insulin cells or somatostatin cells. Large ducts showed a high rate of cell renewal in comparison with small ducts.
Conclusion:
The results of this study indicate that cell renewal rates of the pancreas are not homogeneous, but heterogeneous.
