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Quantities of receptor molecules for colony stimulating factors on leukocytes in measles
You Jeong Kim1, So-Young Kim, Young-Yoo Kim
1Department of Pediatrics, Catholic University Medical College, Seoul, Korea.
Abstract:
We analyzed the comparative amounts of granulocyte-colony stimulating factor (G-CSFr) and granulocyte macrophage CSF (GM-CSFr) receptors expressed on neutrophils and monocytes in measles patients to investigate the role of these CSFrs in the development of leukopenia including neutropenia and monocytopenia in measles. EDTA-anticoagulated peripheral blood of 19 measles patients, 10 children with other infections showing leukopenia and 16 children with normal complete blood cell counts (CBC)s were analyzed using flow cytometry and QuantiBRITE. The leukocyte (5260 +/- 2030/uL vs. 9900 + 2680/uL, p=0.000), neutrophil (2580 +/- 960/uL vs. 4250 +/- 2750/uL, p=0.024) and the lymphocyte counts of measles patients (1810 +/- 1430/uL vs. 4530 +/- 3450/uL, p= 0.006) were lower than in the normal controls. The neutrophils of measles patients expressed similar amounts of G- CSFr (1858 +/- 355) as normal children (1764 +/- 477, p= 0.564) and leukopenic patients (1773 +/- 673, p=0.713), but lower levels of GM-CSFr (535 +/- 118) than normal children (957 +/- 344, p=0.000) and leukopenic patients (832 +/- 294, p=0.002). The monocytes of measles patients expressed similar amounts of G-CSFr (916 +/- 336) and GM-CSFr (3718 +/- 906) as normal children (1013 +/- 391 and 4125 (2645, p > 0.05) but less than leukopenic patients (1454 +/- 398 and 5388 +/- 806, p > 0.05). The neutrophil and monocyte counts of measles patients did not correlate with the amount of G-CSFr or GM-CSFr expressed on neutrophils or monocytes (p > 0.05), but in the normal children, the monocyte count correlated with the levels of GM-CSFr on monocytes (r=0.951, p=0.049). In conclusion, neutropenia is one of the more important characteristics of measles patients, which could be due to the decreased GM-CSFr expression on neutrophils. However, the monocytopenia found in measles patients is not due to the decreased expression of CSFr on the monocytes.
Insights
Measles patients exhibit neutropenia, potentially due to decreased granulocyte macrophage colony-stimulating factor receptor (GM-CSFr) on neutrophils. Monocytopenia in measles is not linked to reduced CSFr expression on monocytes.
Area of Science:
- Immunology
- Hematology
- Virology
Background:
- Measles infection is associated with leukopenia, including neutropenia and monocytopenia.
- Colony-stimulating factor receptors (CSFrs) play a crucial role in regulating neutrophil and monocyte production and function.
Purpose of the Study:
- To investigate the expression of granulocyte-colony stimulating factor receptor (G-CSFr) and granulocyte macrophage CSF receptor (GM-CSFr) on neutrophils and monocytes in measles patients.
- To determine the role of CSFr expression in the development of leukopenia in measles.
Main Methods:
- Flow cytometry and QuantiBRITE were used to analyze CSFr expression on peripheral blood neutrophils and monocytes.
- Blood samples were collected from measles patients, children with other infections causing leukopenia, and healthy controls.
Main Results:
- Measles patients showed significantly lower leukocyte, neutrophil, and lymphocyte counts compared to normal controls.
- Neutrophils from measles patients had similar G-CSFr levels but lower GM-CSFr levels compared to controls.
- Monocyte counts in measles patients did not correlate with G-CSFr or GM-CSFr expression, suggesting monocytopenia is not due to reduced CSFr expression.
Conclusions:
- Neutropenia in measles may be attributed to decreased GM-CSFr expression on neutrophils.
- Monocytopenia in measles patients is not explained by reduced expression of G-CSFr or GM-CSFr on monocytes.