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Expression of mucosal cyto-chemokine mRNAs in patients with Helicobacter pylori infection

S M Park1, J H Kim, Y H Hong

  • 1Department of Internal Medicine, College of Medicine, Chungang University, Seoul, Korea.

The Korean Journal of Internal Medicine
|February 22, 2002
PubMed
Abstract

Insights

Helicobacter pylori infection increases expression of certain chemokines (IL-8, GRO-alpha, MIP-1 alpha, RANTES) in gastric mucosa. These inflammatory markers decrease after successful H. pylori eradication.

Area of Science:

  • Gastroenterology
  • Immunology
  • Molecular Biology

Background:

  • Helicobacter pylori infection damages the gastroduodenal mucosal barrier through inflammatory cell infiltration.
  • Cytokines and chemokines are implicated in the migration and activation of these inflammatory cells.
  • Investigating cyto-chemokine mRNA expression provides insight into H. pylori-induced mucosal changes.

Purpose of the Study:

  • To examine the expression rates of various cytokine and chemokine mRNAs in gastric mucosal biopsy specimens from H. pylori-infected patients.
  • To determine the role of specific inflammatory mediators in H. pylori-associated gastroduodenal pathology.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) was used to analyze mRNA expression in 98 H. pylori-infected patients.
  • Examined mRNA expression of cytokines (IL-1 beta, IL-6, IL-10) and chemokines (MIP-1 alpha, MIP-1 beta, MCAF, RANTES, IL-8, GRO-alpha).

Main Results:

  • Significantly increased mRNA expression of IL-8, GRO-alpha, MIP-1 alpha, and RANTES was observed in H. pylori-positive patients compared to H. pylori-negative patients.
  • No significant difference in mRNA expression of IL-1 beta, IL-6, and IL-10 between the two groups.
  • Following H. pylori eradication, significant decreases in mRNA expression for IL-1 beta, IL-6, IL-10, MIP-1 alpha, MIP-1 beta, MCAF, RANTES, IL-8, and GRO-alpha were noted.

Conclusions:

  • C-X-C chemokines (IL-8, GRO-alpha) and certain C-C chemokines (MIP-1 alpha, RANTES) are significantly upregulated in H. pylori infection.
  • These chemokines likely play a crucial role in the pathogenesis of H. pylori-associated peptic ulcer diseases.
  • Successful eradication of H. pylori leads to a downregulation of these inflammatory mediators.

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