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Related Experiment Videos

Multiple heparanases are expressed in polymorphonuclear cells.

Mary Ann Kosir1, Patricia A Foley-Loudon, Raphaela Finkenauer

  • 1Surgical Service, VA Medical Center, Detroit, Michigan 48201, USA. mary.kosir@med.va.gov

The Journal of Surgical Research
|February 22, 2002
PubMed
Summary
This summary is machine-generated.

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Human neutrophils express both connective tissue-activating-peptide (CTAP-III) and mammalian heparanase (hpa-1). While both proteins are present in similar amounts, hpa-1 exhibits significantly higher heparanase activity, suggesting neutrophils can degrade diverse heparan sulfate chains.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Heparan sulfate proteoglycans are crucial cell surface molecules.
  • Heparanases, found in lysosomes, platelet granules, and neutrophils, degrade heparan sulfate.
  • Two main groups of heparanases exist: CTAP-III and mammalian heparanase (hpa).

Purpose of the Study:

  • To quantify the expression of CTAP-III and hpa in neutrophils.
  • To assess the heparanase activity of CTAP-III and hpa in neutrophils.

Main Methods:

  • Neutrophils were isolated, and RNA was extracted for quantitative RT-PCR to detect CTAP-III and hpa-1 mRNA.
  • Western blot analysis was performed on neutrophil lysate to detect CTAP-III-like and hpa-1 proteins.
  • Chromatography was used to isolate components for heparanase activity assays.

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Main Results:

  • Both CTAP-III and hpa-1 mRNA were equally expressed in neutrophils.
  • CTAP-III-like protein (80 kDa) and hpa-1 (50 kDa) were detected at similar expression levels.
  • Hpa-1 demonstrated significantly higher heparanase activity (1.51 x 10(-6) micromol/min) compared to CTAP-III (0.85 x 10(-6) micromol/min).

Conclusions:

  • Human neutrophils express both CTAP-III-like protein and hpa-1 at the mRNA and protein levels.
  • Hpa-1 exhibits greater heparanase activity than the CTAP-III-like protein.
  • The presence of multiple heparanase classes in neutrophils suggests a role in degrading various heparan sulfate chains.