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Related Experiment Videos

Microbial contamination of cord blood stem cells.

A Honohan1, H Olthuis, A T Bernards

  • 1Sanquin Blood Supply Foundation, Blood Bank Leiden-Haaglanden, Leiden, The Netherlands. aine.honohan@sanquinbblh.nl

Vox Sanguinis
|February 22, 2002
PubMed
Summary

Bacterial contamination in cord blood (CB) is approximately 13%, with low levels (<1 CFU/ml). A 20 ml sample volume is optimal for detecting low-level bacterial contamination in CB units.

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Area of Science:

  • Hematology
  • Microbiology
  • Transfusion Medicine

Background:

  • Low-level bacterial contamination in blood components can lead to sepsis.
  • The significance of bacterial contamination in cord blood (CB) and optimal detection methods are not well-defined.

Purpose of the Study:

  • To investigate bacterial behavior during CB processing.
  • To determine the optimal method for detecting low-level bacterial contamination in CB.

Main Methods:

  • Bacterial spiking experiments were performed on CB units.
  • Bacteria behavior was assessed during centrifugation, freezing, and thawing.
  • Different sample volumes (10ml vs. 20ml) were compared for pathogen detection.

Main Results:

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  • Bacterial contamination in CB units was approximately 13% (<1 CFU/ml).
  • Centrifugation did not concentrate bacteria in specific fractions.
  • DMSO and freezing did not impact bacterial viability.
  • A 20ml sample volume demonstrated higher sensitivity for detecting low-level contamination.

Conclusions:

  • A 20ml waste fraction sample volume is recommended for routine CB bacterial testing.
  • Low-level bacterial contamination in CB is generally not clinically significant unless the blood is expanded ex vivo.
  • The findings provide guidance for improving CB safety and quality control.