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Updated: Oct 2, 2026
Creating Anatomically Accurate and Reproducible Intracranial Xenografts of Human Brain Tumors
Published on: September 24, 2014
Antitumor effect of an adeno-associated virus vector containing the human interferon-beta gene on experimental
Jun Yoshida1, Masaaki Mizuno, Norimoto Nakahara
1Department of Neurosurgery, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya 466-8550, Japan. jyoshida@med.nagoya-u.ac.jp
Abstract:
We constructed an adeno-associated virus (AAV) vector containing the human interferon-beta (HuIFN-b ) gene (AAV-IFN-beta) and investigated its antitumor effect against human glioma cells (U251-SP) inoculated into the brain of nude mice. Prior to this, we examined human glioma cells transduced with AAV-IFN-beta using video-enhanced contrast differential interference contrast (VEC-DIC) microscopy. Infection of AAV-IFN-beta induced apoptosis and secondary necrosis in human glioma cells. In in vivo experiments, we confirmed production of HuIFN-beta and induction of heat-shock protein (HSP) in glioma cells transduced with AAV-IFN-beta. Growth of the experimental gliomas was completely inhibited by six injections of AAV-IFN-beta, starting 7 days after transplantation of glioma cells. In addition, the survival of mice treated with AAV-IFN-beta was remarkably prolonged. These results indicate that AAV-IFN-beta induces apoptosis of glioma cells and has a strong antitumor effect in this experimental glioma model.
