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Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones
Published on: November 30, 2016
[Clone of a novel liver cancer associated gene and analysis of the secondary structures of the predicted protein]
Zhengxu Wang1, Guifang Hu, Hongyang Wang
1Department of General Surgery of Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, China.
Objective:
To clone a novel liver cancer associated gene, and to explore the molecular basis of liver cancer genesis.
Methods:
Using mRNA differential display polymerase chain reaction (DDPCR) and screening the human placenta cDNA library, we got a full-length cDNA of the gene. We prepared and purified the GST fusion protein and the special polyclonal antibody, engaged in the Western blot and immunohistochemical staining analysis, and analyzed the second structures and predicted the function of the protein by the computer soft.
Results:
We have got a full-length cDNA of the liver cancer associated gene and identified that the full-length cDNA of the gene could be expressed in 293 eukaryocytes by Western blot assay. We localized the target protein in cytoplasm using the immunohistochemical staining methods, and found two SH3 binding domains and several protein kinase phosphorylation sites by analyzing the second structures.
Conclusions:
We have got a novel full-length cDNA of human liver cancer associated gene.
Insights
Researchers cloned a novel liver cancer associated gene using mRNA differential display polymerase chain reaction (DDPCR). This discovery aids in understanding liver cancer development.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- Liver cancer genesis involves complex genetic alterations.
- Identifying novel genes associated with liver cancer is crucial for understanding its molecular basis.
Purpose of the Study:
- To clone a novel gene linked to liver cancer.
- To investigate the molecular mechanisms underlying liver cancer development.
Main Methods:
- Utilized mRNA differential display polymerase chain reaction (DDPCR) to identify candidate genes.
- Screened a human placenta cDNA library to obtain the full-length gene.
- Employed Western blot, immunohistochemical staining, and bioinformatic analysis for protein characterization.
Main Results:
- Successfully cloned a full-length cDNA of a novel liver cancer-associated gene.
- Confirmed gene expression in 293 eukaryocytes via Western blot.
- Localized the protein to the cytoplasm and identified SH3 binding domains and kinase phosphorylation sites.
Conclusions:
- A novel full-length cDNA of a human liver cancer-associated gene has been identified.
- The identified protein possesses structural features suggesting roles in cellular signaling pathways relevant to cancer.
