Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glomerular-specific gene excision in vivo.

Vera Eremina1, Mark Andrew Wong1, Shiying Cui1

  • 1*The Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto, Ontario, Canada; and Division of Nephrology, St. Michael's Hospital, Toronto, Ontario, Canada.

Journal of the American Society of Nephrology : JASN
|February 22, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription factor 21 deletion from podocyte precursors as a model for congenital nephrotic syndrome.

American journal of physiology. Renal physiology·2026
Same author

An engineered CRM197 variant with enhanced stability and efficacy.

International journal of biological macromolecules·2026
Same author

Endothelial prolyl hydroxylase 3 mitigates maladaptive inflammation to promote post-ischemic kidney repair.

Kidney international·2026
Same author

A Highly Active Angiopoietin 1 Mimetic Potentiates Angiogenesis in Mouse Models of Choroidal Neovascularization.

Translational vision science & technology·2026
Same author

The transcription factor Tcf21 is necessary for adoption of cell fates by <i>Foxd1</i><sup>+</sup> stromal progenitors during kidney development.

American journal of physiology. Renal physiology·2026
Same author

Piezo1 Activates an Autocrine Angiopoietin-2-Integrin Signaling Loop in Schlemm's Canal to Regulate Intraocular Pressure.

bioRxiv : the preprint server for biology·2025

Researchers developed a new mouse model to study podocyte (kidney filter cells) function. This tool allows specific gene manipulation in podocytes, aiding understanding of kidney diseases like nephrotic syndrome.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genetics

Background:

  • Podocytes are specialized kidney cells crucial for the glomerular filtration barrier.
  • Genetic discoveries have illuminated podocyte slit diaphragm molecules, but many gene functions remain unknown.
  • Studying gene function in specific kidney cell types is challenging due to embryonic lethality or broad effects.

Purpose of the Study:

  • To create a tool for targeted gene manipulation specifically within podocytes.
  • To enable in vivo investigation of gene function in podocytes.
  • To advance understanding of podocyte biology and associated kidney diseases.

Main Methods:

  • Generation of a glomerular-specific Cre-recombinase transgenic mouse line (Neph-Cre) using the Nphs1 (nephrin) promoter.

Related Experiment Videos

  • Utilizing Cre-mediated recombination to excise 'floxed' transgenes.
  • In vivo validation of transgene excision specifically in podocytes.

    • Main Results:

    • Successful generation of the Neph-Cre transgenic mouse line.
    • Demonstrated podocyte-specific Cre-mediated excision of a floxed transgene in vivo.
    • Established a reliable method for genetic manipulation in adult podocytes.

    Conclusions:

    • The Neph-Cre mouse line is a powerful new tool for studying podocyte biology.
    • This model facilitates the investigation of gene function in podocytes in the context of the whole animal.
    • Advances in understanding podocyte function will aid in diagnosing and treating kidney diseases.