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Urinary 19-norandrosterone purification by immunoaffinity chromatography: application to gas
M C Desroches1, J C Mathurin, Y Richard
1Laboratoire National de Dépistage du Dopage, 143 avenue Roger Salengro, 92290 Châtenay-Malabry, France.
Abstract:
The detection of exogenous 19-norandrosterone (19-NA) in urines was investigated by using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). 19-NA is, for the first time to our knowledge, isolated from urinary matrix by specific immunoaffinity chromatography (IAC) before analysis. The sample preparation consisted of a preliminary purification of urine by solid-phase extraction after hydrolysis by beta-glucuronidase. Unconjugated 19-NA was thus isolated by IAC and directly analysed by GC/C/IRMS. Optimisation of IAC purification was achieved and the reliability of the technique for anti-doping control is discussed.