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Related Experiment Videos

Comparative proteomics based on stable isotope labeling and affinity selection.

Fred E Regnier1, Larry Riggs, Roujian Zhang

  • 1Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. fregnier@purdue.edu

Journal of Mass Spectrometry : JMS
|February 22, 2002
PubMed
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New internal standard technology enables precise quantification of protein changes in biological samples. Stable isotope labeling and mass spectrometry help identify disease-specific protein markers for diagnostic use.

Area of Science:

  • Proteomics
  • Biochemistry
  • Molecular Biology

Background:

  • Biological systems regulate protein levels through synthesis, modification, transport, and degradation.
  • Identifying specific proteins affected by disease or stimuli is challenging due to sample complexity and diverse post-translational modifications.

Purpose of the Study:

  • To introduce novel tools and methods for global peptide quantification using internal standard technology.
  • To leverage stable isotope labeling and mass spectrometry for targeted protein analysis.

Main Methods:

  • Development of internal standard technology for coding and quantifying peptides in biological samples.
  • Application of stable isotope-labeling strategies for mass spectrometry-based protein analysis.
  • Utilizing chromatographic selection based on specific structural features for targeted peptide analysis.

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Main Results:

  • Stable isotope-labeling strategies enable rapid targeting of proteins with altered concentrations.
  • Targeted peptide analysis, focusing on unique structural features, aids in identifying regulatory or disease-specific proteins.
  • Differential displays of targeted peptides reveal stimulus-specific markers.

Conclusions:

  • Novel internal standard technologies facilitate comprehensive protein quantification.
  • Targeted peptide analysis combined with stable isotope quantification is effective for identifying disease and stimulus markers.
  • Identified markers show potential as valuable diagnostic tools.