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Updated: Oct 2, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
[Molecular mechanism of hormone-dependent transcriptional regulation of nuclear receptor]
Kazuhiro Ikeda1, Satoushi Inoue
1Research Center for Genomic Medicine, Saitama Medical School.
Abstract:
Various actions of steroids, thyroid, vitamin D and retinoic acid are mediated by cognate nuclear hormone receptors. Hormone-liganded receptors bind to hormone response element in transcription regulatory region and homodimerize or heterodimerize with retinoid X receptor and regulate transcription of target genes. Conformational change of nuclear receptor after ligand-binding gives association with coactivators which possess histone acetyltransferase activity and are necessary for hormone-dependent transactivation. In the absence of hormone, corepressors, which associate with histone deacetyltransferase, interact to nuclear hormone receptors and repress the transcription. All these factors have important roles in the signaling of nuclear receptor and may be involved in endocrine dysfunction and disease.
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