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Updated: Oct 2, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
[The etiology of steroid hormone insensitivity: nuclear cofactors and subnuclear architecture]
1Department of Geriatric Medicine, Graduate School of Medical Sciences, Kyushu University.
Abstract:
Steroid hormone receptors interact with the basal transcription machinery(BTM) via intermediary cofactor complexes. Two complexes, a CBP/p300 complex with histone acetyltransferase(HAT) and a TRAP/DRIP/ARC/SMCC complex without HAT are identified, and a multistep model is proposed in which the two complexes interact sequentially with nuclear receptors. Recent advances in live-cell imaging techniques have revealed the interrelationship between subnuclear architecture and gene transcription, especially, nuclear receptors are known to form foci in transcriptionally activated state. These advances have developed a novel concept in a disorder of steroid hormone insensitivity. Disruption of the transmission from the AF-1 domain of the androgen receptor to BTM was revealed in a patient with androgen insensitivity who has no mutations in the androgen receptor gene.
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