Rapid diagnosis of enterovirus infections by real-time PCR on the LightCycler using the TaqMan format
Related Experiment Video
Updated: Aug 9, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Thomas Watkins-Riedel1, Markus Woegerbauer, David Hollemann
1The Division of Clinical Virology, Institute of Virology and Infectious Diseases, University Hospital Vienna, A-1090, Vienna, Austria. thomas.watkins-riedel@akh-wien.ac.at
Abstract:
A 2 h single-tube, reverse-transcription(RT)-PCR/hybridization assay using the TaqMan format for rapid diagnostic screening of enterovirus (EV) infections was optimized for the real-time LightCycler (LC) technology. For low EV load clinical samples an additional 30 min reamplification step using a novel primer-mix/probe design resulted in a 100% concordance with AMPLICOR EV PCR Test and in-house RT-PCR. Combined with maximum specificity, the sensitivity of LC-PCR was 10- to 100-fold higher compared to AMPLICOR EV Test.
More Related Videos
12:32EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
Published on: January 16, 2016
08:36Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018