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Investigation of calcium-binding proteins using electrospray ionization mass spectrometry

Amanda L Doherty-Kirby1, Gilles A Lajoie

  • 1Department of Biochemistry, University of Western Ontario, London, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2002
PubMed
Abstract

No abstract available in PubMed .

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Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

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