Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Does antisense make sense in dermatology?

P E van Erp1, M Wingens

  • 1Department of Dermatology, University Medical Center Nijmegen, The Netherlands. p.vanerp@derma.azn.nl

Acta Dermato-Venereologica
|February 28, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment of psoriasis with a new combination of calcipotriol and betamethasone dipropionate: a flow cytometric study.

Dermatology (Basel, Switzerland)·2001
Same author

In situ demonstration of phosphorylated c-jun and p38 MAP kinase in epidermal keratinocytes following ultraviolet B irradiation of human skin.

The Journal of pathology·2001
Same author

Cell-kinetic evidence for increased recruitment of cycling epidermal cells in psoriasis: the ratio of histone and Ki-67 antigen expression is constant.

Dermatology (Basel, Switzerland)·2000
Same author

Changes in keratin 6 and keratin 10 (co-)expression in lesional and symptomless skin of spreading psoriasis.

Dermatology (Basel, Switzerland)·2000
Same author

Flow-cytometric characterization of normal versus psoriatic epidermis using improved cell separation methodology.

Experimental dermatology·2000
Same author

CD 2394, a novel synthetic retinoid, initiates an embryonic type of differentiation in hyperproliferative skin.

Acta dermato-venereologica·2000

Antisense technology uses short DNA sequences to block specific gene activity, offering potential for treating skin diseases. While promising for targeted gene modulation in the skin, this approach is still in early development.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Dermatology

Background:

  • Antisense technology utilizes short oligodeoxynucleotides (ODNs) that bind to messenger RNA (mRNA) via Watson-Crick base pairing.
  • This binding interferes with protein synthesis, offering a sequence-specific method to modulate gene expression.
  • The skin's accessibility makes it a potential target for topical antisense ODN therapies.

Purpose of the Study:

  • To review the utility of antisense ODN technology for modulating gene expression in skin diseases.
  • To assess the potential of targeted inhibition of keratinocyte gene expression for dermatological treatment.
  • To provide insights into the practical application and current limitations of antisense technology in dermatology.

Main Methods:

  • Review of recent studies on antisense ODN applications in skin models.

Related Experiment Videos

  • Analysis of the principles of DNA-RNA hybrid formation and gene silencing.
  • Evaluation of experimental data regarding the efficacy and specificity of antisense ODNs in dermatological contexts.

    • Main Results:

    • Antisense ODNs can specifically inhibit gene expression in keratinocytes.
    • Successful application of antisense technology has been demonstrated in some skin model systems.
    • Careful experimentation and critical data interpretation are essential for practicing antisense technology.

    Conclusions:

    • Antisense ODN technology holds promise for treating skin diseases by modulating gene expression.
    • Despite successes in model systems, the technology is considered to be in its early stages.
    • Further research is required to address fundamental questions before widespread clinical application in dermatology.