Random sequence libraries displayed on phage: identification of biologically important molecules
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Updated: Oct 2, 2026

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Published on: October 3, 2018
Christopher G Adda1, Robin F Anders, Leann Tilley
1Department of Biochemistry, and the Cooperative Research Centre for Vaccine Technology, La Trobe University, Bundoora, 3083, Victoria, Australia.
Abstract:
Phage display has become a widely used tool for the identification of proteins or peptides with affinity for a variety of biomolecules. The versatility, simplicity and cost effectiveness of this application has pervaded a wide variety of research areas. Although not without its limitations, phage display has provided a convenient methodology for obtaining ligands to study the function, structure and diagnostic or therapeutic potential of various macromolecules. This review highlights some recent research employing this technology that serves to illustrate its utility in various research and clinical applications.
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