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Related Experiment Videos

A tissue-engineered stent for cell-based vascular gene transfer.

Carmelo J Panetta1, Katsumi Miyauchi, David Berry

  • 1Division of Cardiovascular Diseases and Molecular Medicine Program, Mayo Clinic, Rochester, MN 55905, USA.

Human Gene Therapy
|February 28, 2002
PubMed
Summary
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This study developed a novel stent for stable, site-specific gene transfer in blood vessels. The fibronectin-coated stent successfully maintained green fluorescence protein (GFP) expression in smooth muscle cells for 4 weeks post-implantation.

Area of Science:

  • Biomedical Engineering
  • Vascular Biology
  • Gene Therapy

Background:

  • Site-specific gene expression in the vasculature is challenging.
  • Stent-based platforms offer potential for localized therapeutic delivery.

Purpose of the Study:

  • To develop and evaluate a novel stent platform for stable, long-term cell-based gene transfer in the vasculature.
  • To assess in vivo transgene expression and safety of the gene-modified stent.

Main Methods:

  • A fibronectin-coated mesh-stent was developed.
  • Autologous porcine smooth muscle cells (SMC) were transduced with a green fluorescence protein (GFP) plasmid.
  • Gene-modified SMC were seeded onto the stent and implanted in porcine coronary arteries.
  • In vivo GFP expression, cell numbers, and histological/angiographic analyses were performed over 4 weeks.

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Main Results:

  • Stable in vivo GFP expression (5.2 x 10(5) GFP-positive cells/cm(2) mesh) was achieved and maintained for 1 month.
  • No significant decrease in GFP-positive cell number was observed over the 1-month period.
  • Angiography and histology showed mild neointimal proliferation with no inflammatory infiltrate.

Conclusions:

  • The novel stent platform enables stable, site-specific, long-term transgene expression in the vasculature.
  • This approach holds promise for treating cardiovascular and other diseases requiring sustained cell-based gene delivery.