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Updated: Oct 2, 2026
An Electroporation Method to Transform Rickettsia spp. with a Fluorescent Protein-Expressing Shuttle Vector in Tick Cell Lines
Published on: October 11, 2022
[Isolation and identification of HN-98 strain of spotted fever group rickettsiae]
1Department of Rickettsiology, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing, 102206, China.
Objective:
One strain of rickettsiae was isolated from a patient with unknown fever from Qiongzhong county in Hainan Province in 1998, using embryonated hen eggs and proved to be the member of rickettsiae by the methods of morphology. It was named HS-98 strain after the name of the place and the year it was isolated.
Methods:
The isolate was identified by methods of Microcomplement fixation assay and OCR/RFLP and compared with known species and strains of SFGR by DNA polymerase chain reaction and DNA polymerase chainreaction and was followed by restriction endonuclease fragment length polymorphism analysis.
Results:
Results demonstrated that HN-98 strain was antigenically and genotypically identical to R. sibirica 246.
Conclusion:
The results suggested that there existed natural foci of spotted fever in Qiongzhong Area of Hainan Province.
