Related Experiment Video
Updated: Jan 1, 2026
In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration
Published on: February 23, 2019
[Effect of inorganic arsenic on gap junctional intercellular communication between human skin fibroblasts]
1Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing 100083, China.
Objective:
The aim of this study is to investigate the effect of inorganic arsenic on gap junctional intercellular communication (GJIC) between human skin fibroblasts.
Methods:
GJIC between human skin fibroblasts was detected by the scrape loading/dye transfer assay.
Results:
Arsenite at concentrations from 0.005 micromol/L to 5.0 micromol/L significantly inhibited the dye transfer between human skin fibroblasts in a dose dependent manner. The dye transfer between human skin fibroblasts was also inhibited by arsenate, while the dose-dependent effects were not observed. Further study found that arsenite at concentrations of 0.5 micromol/L and 5.0 micromol/L significantly increased protein kinase C (PKC) activities in cells, especially in the membrane fraction.
Conclusions:
Arsenite and arsenate significantly inhibited GJIC between human skin fibroblasts, suggesting that inorganic arsenic may play an important role in the process of tumor promotion. Furthermore, PKC activation may be involved in the mechanisms of GJIC inhibition by inorganic arsenic.
More Related Videos
05:27Gap Junctional Intercellular Communication: A Functional Biomarker to Assess Adverse Effects of Toxicants and Toxins, and Health Benefits of Natural Products
Published on: December 25, 2016
11:02A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide
Published on: October 18, 2014
Related Concept Videos
01:17Intracellular Signaling Affects Focal Adhesions
Some...
01:24Overview of Cell-Matrix Interactions