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Chemical incorporation of 1-methyladenosine into oligonucleotides
Sergey N Mikhailov1, Jef Rozenski, Ekaterina V Efimtseva
1Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.
Nucleic Acids Research
|February 28, 2002
Abstract:
The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.