Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

From stem cells to prion signalling.

Odile Kellermann1, Claire Lafay-Chebassier, Myriam Ermonval

  • 1Laboratoire de différenciation cellulaire et prions, CNRS UPR 1983-Institut Pasteur, BP 8, 94801 Villejuif, France. okellerm@vjf.cnrs.fr

Comptes Rendus Biologies
|February 28, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beyond amyloid and tau: synaptic and neurodegenerative biomarkers shape MCI progression.

Molecular psychiatry·2026
Same author

Tau profiling of brain extracellular vesicles reveals PHF6 peptide as core for pathological tau seeding in Alzheimer's disease.

Journal of biomedical science·2026
Same author

Blood Neurofilament Light Chain and Glial Fibrillary Acidic Protein as Candidate Biomarkers in <i>CSF1R</i>-Related Disorder.

Neurology. Genetics·2026
Same author

Influence of Decreased Kidney Function on Plasma Biomarkers of Neurodegenerative Disorders in Routine Care: Confirmation of the Interest of Ratios.

Neurology·2026
Same author

From clusters to clinic: An 8-gene signature combined with mucinous component stratifies benefit of anti-CTLA-4 addition to anti-PD-1 in dMMR/MSI-H metastatic colorectal cancer.

European journal of cancer (Oxford, England : 1990)·2026
Same author

Unhealthy food bias in food addiction: behavioral evidence from a probabilistic image choice task.

Journal of eating disorders·2026
Same journal

Integrating intraspecific trait variation and spatiotemporal variability of selection as levers of action in forest management.

Comptes rendus biologies·2026
Same journal

[Managing mixed forest stands : bridging the gap between silviculture and ecological theory].

Comptes rendus biologies·2026
Same journal

[Which forests for which biodiversity under climate change?]

Comptes rendus biologies·2026
Same journal

[Impacts of climate change on the functioning and productivity of agroecosystems: a focus on the impact of interactions between CO<sub>2</sub>, temperature and water deficit].

Comptes rendus biologies·2026
Same journal

Autogenic transitions in individuality.

Comptes rendus biologies·2026
Same journal

Assessing the potential effects of climate change on future forest composition in France.

Comptes rendus biologies·2026
See all related articles

This study introduces a novel immortalized neural stem cell line (1C11) that differentiates into specific neuron types. Researchers found that prion protein (PrPc) signaling fine-tunes neuronal functions and may be a target for prion disease research.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Stem Cell Research

Background:

  • The 1C11 cell line, derived from multipotential cells, serves as a neuroepithelial progenitor.
  • These cells can differentiate into serotonergic or noradrenergic neurons, regulated by specific receptors.

Purpose of the Study:

  • To investigate the role of prion protein (PrPc) in neuronal differentiation and function.
  • To explore the potential of the 1C11 cell line as a model for studying prion diseases.

Main Methods:

  • Immortalization of multipotential cells using an adenovirus-SV40 plasmid.
  • Induction of neuronal differentiation in 1C11 cells.
  • Analysis of PrPc expression, localization, and interaction with Fyn kinase and caveolin-1.

Related Experiment Videos

Main Results:

  • 1C11 cells differentiate into nearly 100% serotonergic or noradrenergic neurons upon induction.
  • PrPc is constitutively expressed and its cross-linking activates Fyn kinase in a caveolin-1 dependent manner.
  • PrPc-Fyn coupling is observed in differentiated neurons, primarily at neurites, suggesting a role in signal transduction.

Conclusions:

  • Prion protein (PrPc) acts as a signal transduction protein, potentially fine-tuning neuronal functions.
  • The 1C11 cell line, capable of prion replication, offers a valuable tool to study the impact of PrPSc accumulation on PrPc signaling.