Related Experiment Video
Updated: Aug 15, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
A syringe electrode device for simultaneous injection of DNA and electrotransfer
1Center for Pharmacogenetics, School of Pharmacy, University of Pittsburgh, 633 Salk Hall, Pittsburgh, Pennsylvania 15213, USA.
Abstract:
Electroporation for gene delivery has attracted considerable attention recently, because of both the site-specific nature of the delivery and the high efficiency of the method. Electrotransfer of genes involves the application of an electric field to cells to enhance their permeability, facilitating exogenous polynucleotide transit across the cytoplasmic membrane. However, the relatively high electric field strength required for electroporation induces tissue damage, thus limiting its widespread application. We describe here a syringe electrode of our design, with which the same transfection efficiency can be achieved by using much lower electric field strength than that of conventional electrodes, such that the tissue damage is minimized.
More Related Videos
07:52Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain
Published on: July 7, 2015
06:32Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles
Published on: September 4, 2019
Related Concept Videos
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Electrodeposition
Electrodeposition can...
Capillary Electrophoresis: Instrumentation