Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altering retroviral tropism using a random-display envelope library.

Keith Bupp1, Monica J Roth

  • 1Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, New Jersey 08854, USA.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|February 28, 2002
PubMed
Summary

Researchers developed a novel retroviral envelope protein for targeted gene delivery. This engineered protein specifically targets canine osteosarcoma cells, offering a promising tool for gene therapy applications in large animal models.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loop Plasticity Drives Paralog-Specific Recognition in BET ET Domains.

Journal of chemical information and modeling·2026
Same author

Correction: Hybrid AI/physics pipeline for miniprotein binder prioritization: application to the BRD3 ET domain.

Chemical communications (Cambridge, England)·2026
Same author

Loop Plasticity Drives Paralog-Specific Recognition in BET ET Domains.

bioRxiv : the preprint server for biology·2025
Same author

Hybrid AI/physics pipeline for miniprotein binder prioritization: application to the BRD3 ET domain.

Chemical communications (Cambridge, England)·2025
Same author

Memorization bias impacts modeling of alternative conformational states of solute carrier membrane proteins with methods from deep learning.

PLoS computational biology·2025
Same author

Memorization Bias Impacts Modeling of Alternative Conformational States of Symmetric Solute Carrier Membrane Proteins with Methods from Deep Learning.

bioRxiv : the preprint server for biology·2024

Area of Science:

  • Molecular Biology
  • Virology
  • Gene Therapy

Background:

  • Tissue-specific gene delivery is crucial for effective gene therapy.
  • Current methods often lack precise cell targeting capabilities.
  • Retroviral envelope proteins mediate viral entry into host cells.

Purpose of the Study:

  • To develop a novel retroviral envelope protein for cell-specific gene delivery.
  • To identify envelope protein variants with altered host range and receptor usage.
  • To assess the potential of engineered envelope proteins for targeted gene therapy.

Main Methods:

  • Screening a large library (10^6) of feline leukemia virus subgroup A envelope protein variants.
  • Introducing 10 random amino acid substitutions in the receptor-determining region.

Related Experiment Videos

  • Selecting for functional envelope proteins capable of stable gene transfer.
  • Analyzing the host range and receptor usage of identified variants using interference assays.

    • Main Results:

    • Identification of a single envelope protein variant, designated EF, with high specificity for D17 canine osteosarcoma cells.
    • EF demonstrated altered receptor usage compared to parental subgroup A and C envelope proteins.
    • This specificity was not observed with the original subgroup A and C proteins.

    Conclusions:

    • The engineered EF envelope protein enables highly specific gene delivery to canine osteosarcoma cells.
    • This finding represents the first successful demonstration of cell-specific entry via screening retroviral envelope protein libraries.
    • The EF protein is a valuable tool for investigating gene therapy strategies for osteosarcoma in large animal models.