Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Map-based cloning of quantitative trait loci: progress and prospects.

D L Remington1, M C Ungerer, M D Purugganan

  • 1Department of Genetics, North Carolina State University, Raleigh, NC 27695-7614, USA. dlreming@unity.ncsu.edu

Genetical Research
|February 28, 2002
PubMed
Summary

Map-based cloning is now a viable method for isolating quantitative trait loci (QTLs) in plants. Recent successes demonstrate its potential for gene discovery, overcoming previous challenges.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effects of mating design on introgression between chromosomally divergent sunflower species.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Average effect of a mutation in lignin biosynthesis in loblolly pine.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2012
Same author

The plant proteome folding project: structure and positive selection in plant protein families.

Genome biology and evolution·2012
Same author

Permanent Genetic Resources added to the Molecular Ecology Resources Database 1 February 2010-31 March 2010.

Molecular ecology resources·2011
Same author

Transposable elements as introns: evolutionary connections.

Trends in ecology & evolution·2011
Same author

Evolution of development: molecules, mechanisms and phylogenetics.

Trends in ecology & evolution·2011

Area of Science:

  • Genetics
  • Molecular Biology
  • Plant Science

Background:

  • Map-based cloning is traditionally challenging for quantitative trait loci (QTLs) due to environmental and genetic complexities.
  • Previous research has often avoided map-based approaches for QTL isolation.

Purpose of the Study:

  • To review recent successful applications of map-based cloning for QTL isolation in plants.
  • To evaluate the feasibility and prospects of these techniques for broader use.

Main Methods:

  • Analysis of five recent plant studies that successfully employed map-based cloning for QTLs.
  • Identification of key features enabling successful QTL isolation using these methods.

Main Results:

  • Five plant studies have successfully cloned QTLs using map-based methods.

Related Experiment Videos

  • Successful cloning requires QTLs to be single genes isolatable in near-isogenic lines.
  • Accurate genotype inference through progeny testing is crucial.

    • Conclusions:

    • Map-based cloning is a feasible approach for QTL isolation, particularly in plants.
    • Modified procedures for QTLs are manageable when discrete phenotypes are present.
    • Method selection depends on species and trait-specific factors.