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A major decomposition product, citrinin H2, from citrinin on heating with moisture
Mitsuru Hirota1, Alka Mehta, Keiko Yoneyama
1Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, Kami-ina, Nagano, Japan. hirotam@gipmc.shinshu-u.ac.jp
Abstract:
Citrinin is one of the mycotoxins produced by Penicillium citrinum. We examined the decomposition products after heating citrinin in water at 140 degrees C and isolated a major product, citrinin H2 (3-(3,5-dihydroxy-2-methylphenyl)-2-formyloxy-butane). Citrinin H2 did not show significant cytotoxicity to HeLa cells up to a concentration of 200 microg/ml (% cytotoxicity: 39%) in 63 h of incubation, but citrinin showed severe toxicity at a concentration of 25 microg/ml (% cytotoxicity: 73%). HPLC analysis of citrinin after heating under various conditions indicates that citrinin H2 is mainly yielded from citrinin.