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Inhibitory effects of praeruptorin C on cattle aortic smooth muscle cell proliferation
En-Hui Wei1, Man-Ren Rao, Xiu-Ying Chen
1Department of Cardiovascular Pharmacology, Nanjing Medical University, Nanjing 210029, China.
Aim:
To study the effects of praeruptorin C (pra-C) on proliferation of cattle aortic smooth muscle cells (SMC).
Methods:
The DNA synthesis of SMC was measured using the incorporation of [3H]thymidine([3H]TdR). Cell cycle phase was evaluated by flow cytometry and cytotoxicity was evaluated by measuring lactic dehydrogenase (LDH) activity.
Results:
Whether or not treated with angiotensin II (Ang-II), SMC proliferation was suppressed by pra-C in a concentration-dependent manner at range from 0.001 micromol/L to 10 micromol/L. The inhibitory effects appeared to be related to G1-S block in cell cycle traverse while the LDH activities did not change dramatically.
Conclusion:
Pra-C can completely inhibit SMC proliferation induced by Ang II and partly inhibit the growth of SMC- induced by bovine serum, which is important in prevention and treatment of vascular hyperplastic disease.