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Effect of mifepristone on uterine receptivity in guinea pigs
Cheng-Quan Liu1, Zhong-Xing Wang, Yao Yuan
1National Laboratory of Contraceptives and Devices Research, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China. songsi@mail.sippr.stc.sh.cn
Aim:
To determine the effect of a low-dose mifepristone regimen on endometrial receptivity in guinea pigs.
Methods:
Histologic evaluation for endometrial staging and immunoreactivity of cytokines [leukemia inhibitory factor (LIF) and interleukin-6 (IL-6)] were preformed on fixed endometrial tissue, and relative abundance of endometrial estrogen (E), and progesterone (P) receptor mRNA were evaluated with semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) in which cyclophilin mRNA, a housekeeping gene product, was co-amplified as the reference standard.
Results:
Mifepristone at 0.03 mg.kg-1 . d-1 induced a delay in endometrial maturation. The staining intensity of LIF and IL-6 decreased in treated guinea pigs. E and P receptor mRNA increased significantly and uterine stage delayed in treated guinea pigs.
Conclusion:
Mifepristone at 0.03 mg.kg-1 . d-1 delayed development of endometrium. This study provides further evidence that endometrial maturation can be altered without affecting ovarian ovulatory function.
