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Genomic instability at the human CD5 gene promoter
Agustí López-de la Iglesia1, Javier Calvo, Llúcia Sanz-Vaqué
1Institut Clínic d'Infeccions i Immunologia, Institut D'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic, Barcelona, Spain.
Haematologica
|March 1, 2002
Summary
The CD5 microsatellite (MS) is a valuable marker for genome instability. It showed the highest frequency of abnormalities in B-cell chronic lymphocytic leukemia (B-CLL) and mantle cell lymphoma (MCL).
Area of Science:
- Genetics and Genomics
- Cancer Biology
- Molecular Oncology
Background:
- The human CD5 gene, located at 11q12.2, is near cancer-associated mutation regions.
- The CD5 gene's 5'-flanking region contains a conserved microsatellite (MS) useful for detecting genome instability.
Purpose of the Study:
- To evaluate the CD5 microsatellite (MS) as a marker for genome instability.
- To specifically assess its utility in B-cell leukemia and lymphoma.
Main Methods:
- Analyzed CD5 MS and 10 other MS markers using polymerase chain reaction (PCR) and sequencing gels.
- Studied cell lines and patient samples: 28 B-cell chronic lymphocytic leukemia (B-CLL), 19 mantle cell lymphomas (MCL), and 45 head and neck carcinomas (HNC).
Main Results:
- CD5 MS instability frequency in head and neck carcinomas was comparable to other known MS markers.
- CD5 MS instability was the most frequent abnormality observed in B-cell chronic lymphocytic leukemia (B-CLL) and mantle cell lymphoma (MCL).
Conclusions:
- Microsatellite markers on chromosome 11q, particularly CD5 MS, are highly informative for genome instability analysis.
- These markers are especially relevant for understanding B-cell leukemias and lymphomas.