Continuous protein recovery from whey using liquid-solid circulating fluidized bed ion-exchange extraction
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Updated: Oct 2, 2026

Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
Published on: February 7, 2022
Qingdao Lan1, Amarjeet Bassi, Jing-Xu Jesse Zhu
1Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON, Canada N6A 5B9.
Abstract:
A liquid-solid circulating fluidized bed (LSCFB) continuous ion-exchange extraction system has been investigated for total protein recovery from whey solutions under various operating conditions. The effectiveness of a dynamic seal was evaluated between the riser and the downcomer, and the best conditions for the establishment of this seal were established. Start-up studies indicated that the system is robust and stable. Under optimal conditions, a productivity of 8.2 g of total protein removed per hour per kilogram of resin was achieved with a protein removal efficiency of 78.4%. However, higher overall protein recovery of up to 90% was also achieved under other conditions, with lower protein concentration in the effluent and a lower overall productivity.
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