Nonaqueous capillary electrophoretic separation of basic enantiomers using
M Brent Busby1, Omar Maldonado, Gyula Vigh
1Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA.
Abstract:
The enantiomers of 34 pharmaceutical weak-base analytes were separated by nonaqueous capillary electrophoresis in acidic methanol background electrolytes using the sodium salt of the new, single-isomer chiral resolving agent, octakis(2,3-O-dimethyl-6-O-sulfo)-gamma-cyclodextrin (ODMS). The effective mobilities, separation selectivities and peak resolution values of the weak-base analytes were determined as a function of the ODMS concentration in the 0-40 mM range and were found to follow the theoretical predictions of the charged resolving agent migration model (CHARM model) modified for ionic strength effects. Fast, efficient separations were achieved for both comparatively small and large enantiomers.
More Related Videos
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Racemic Mixtures and the Resolution of Enantiomers
Electrophoresis: Overview
There...
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...


