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Updated: Oct 1, 2026

Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
Micellar electrokinetic chromatography with diode laser-induced fluorescence detection as a tool for investigating
Peng Jing1, Takashi Kaneta, Totaro Imasaka
1Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka, Japan.
Abstract:
We describe the use of micellar electrokinetic chromatography with diode laser-induced fluorometry (MEKC-DLIF) as a tool to characterize a labeling reaction between sulfoindocyanine succinimidyl ester (Cy5), a cyanine fluorescent dye, and a model protein, bovine serum albumin (BSA). To decrease the influence of imprecise injection, methylene blue was added as an internal standard. Labeled BSA was completely separated from the unconjugated Cy5, and methylene blue under optimized conditions. A kinetic study of the reaction was performed by changing some parameters, such as reaction buffer pH, reaction buffer concentration and the initial concentration of BSA. A comparison between the current method and traditional methods was made.
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