Jove
Visualize
Contact Us

Related Experiment Videos

A pit stop at the ER.

Gordon N Gill1

  • 1University of California, San Diego, La Jolla, CA 92093-0650, USA. ggill@ucsd.edu

Science (New York, N.Y.)
|March 2, 2002
PubMed
Summary
This summary is machine-generated.

Cellular signaling deactivation is complex. New research visualizes the dephosphorylation of internalized receptor tyrosine kinases by endoplasmic reticulum phosphatases, revealing a key step in turning off cell signals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Viewing serine/threonine protein phosphatases through the eyes of drug designers.

The FEBS journal·2013
Same author

Bio-molecular architects: a scaffold provided by the C-terminal domain of eukaryotic RNA polymerase II.

Nano reviews·2011
Same author

Structural and functional analysis of the phosphoryl transfer reaction mediated by the human small C-terminal domain phosphatase, Scp1.

Protein science : a publication of the Protein Society·2010
Same author

Islet-to-LMO stoichiometries control the function of transcription complexes that specify motor neuron and V2a interneuron identity.

Development (Cambridge, England)·2009
Same author

Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1.

Molecular cell·2006
Same author

The Grainyhead-like epithelial transactivator Get-1/Grhl3 regulates epidermal terminal differentiation and interacts functionally with LMO4.

Developmental biology·2006
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Cellular Biology
  • Molecular Signaling
  • Biochemistry

Background:

  • Ligand activation of receptor signaling is a fundamental cellular process.
  • Mechanisms for switching off activated receptor signaling are less understood.
  • Receptor tyrosine kinases (RTKs) play crucial roles in cell communication.

Purpose of the Study:

  • To elucidate the deactivation process of ligand-activated receptor tyrosine kinases.
  • To visualize a specific step in turning off RTK signaling.
  • To understand the role of phosphatases in receptor signal termination.

Main Methods:

  • The study visualizes the dephosphorylation of internalized receptors.
  • Focuses on the action of a phosphatase located in the endoplasmic reticulum.

Related Experiment Videos

  • Builds upon the work by Haj et al. as discussed in a Perspective by Gill.

    • Main Results:

    • Visual evidence of receptor dephosphorylation in the endoplasmic reticulum.
    • Identifies a phosphatase as the key enzyme in this deactivation step.
    • Provides insight into the spatial and enzymatic aspects of signal termination.

    Conclusions:

    • Dephosphorylation by endoplasmic reticulum phosphatases is a critical step in deactivating receptor tyrosine kinase signaling.
    • Understanding this deactivation pathway is essential for comprehending cellular signal regulation.
    • This work contributes to the knowledge of how cells manage and terminate signaling events.