Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biological dosimetry using human interphase peripheral blood lymphocytes.

Pataje G S Prasanna1, Christine J C Hamel, Nestor D Escalada

  • 1Applied Cellular Radiobiology Department, Armed Forces Radiobiology Research Institute, 8901 Wisconsin Avenue, Bethesda, MD 20889-5603, USA.

Military Medicine
|March 5, 2002
PubMed
Summary

New interphase-based assays offer faster radiation dose assessment than traditional methods. These cytological techniques, including chromosome aberration and mitochondrial DNA mutation analysis, show promise for biological dosimetry.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Re-envisioning the North American Biodosimetry Assessment Networking Group.

Disaster medicine and public health preparedness·2026
Same author

Activities of ISO Working Group 18: Biological and Physical Retrospective Dosimetry.

Disaster medicine and public health preparedness·2025
Same author

Best practices and novel approaches for the preclinical development of drug-radiotherapy combinations for cancer treatment.

The Lancet. Oncology·2024
Same author

Harnessing Senescence for Antitumor Immunity to Advance Cancer Treatment.

Radiation research·2024
Same author

Older adults with cancer and common comorbidities-challenges and opportunities in improving their cancer treatment outcomes.

Journal of the National Cancer Institute·2024
Same author

Radiation Research Society Journal-based Historical Review of the Use of Biomarkers for Radiation Dose and Injury Assessment: Acute Health Effects Predictions.

Radiation research·2024

Area of Science:

  • Cytogenetics
  • Radiation Biology
  • Molecular Biology

Background:

  • Conventional chromosome-aberration biodosimetry is labor-intensive and time-consuming.
  • Developing rapid, interphase-based assays is crucial for broad radiation exposure scenarios.

Purpose of the Study:

  • To develop and evaluate simple, rapid interphase-based cytological assays for radiation biodosimetry.
  • To establish a dose-effect relationship for gamma irradiation-induced chromosome 1 aberrations.

Main Methods:

  • Utilized premature chromosome condensation-fluorescence in situ hybridization (PCC-FISH) assay on resting human lymphocytes.
  • Quantified "excess spots" on chromosome 1 after gamma irradiation (0-7.5 Gy).
  • Employed in situ polymerase chain reaction for mitochondrial DNA (mtDNA) deletion assay.

Related Experiment Videos

Main Results:

  • A dose-dependent increase in chromosome 1 aberrations ("excess spots") was observed, fitting a nonlinear power model.
  • The number of excess spots per cell increased from 0.035 at 0.5 Gy to 0.236 at 7.5 Gy.
  • The mtDNA deletion assay demonstrated high analyzability (90-95% of cells).

Conclusions:

  • Interphase-based assays, like PCC-FISH and mtDNA deletion analysis, offer advantages over metaphase-spread methods.
  • These assays can overcome limitations such as low analyzable cell counts and extensive labor.
  • The developed techniques show potential for efficient and accurate biological dosimetry and retrospective dose reconstruction.