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Nitric oxide biosensors based on Hb/phosphatidylcholine films
Chunhai Fan1, Jiantao Pang, Pingping Shen
1Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology, Nanjing University, PR China.
Summary
A novel biosensor immobilized hemoglobin (Hb) in a phosphatidylcholine (PC) film on pyrolytic graphite (PG) electrodes. This reagentless sensor effectively detects nitrogen oxide (NO) with high sensitivity and stability.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Biomaterials
Background:
- Hemoglobin (Hb) is a crucial protein for oxygen transport.
- Developing sensitive and selective biosensors for nitrogen oxide (NO) detection is important for various applications.
- Immobilization techniques are key to enhancing biosensor stability and performance.
Purpose of the Study:
- To develop a reagentless biosensor for nitrogen oxide (NO) detection.
- To investigate the electrochemical and enzyme-like activity of hemoglobin (Hb) immobilized in a phosphatidylcholine (PC) film.
- To characterize the performance of the developed Hb/PC/pyrolytic graphite (PG) biosensor.
Main Methods:
- Entrapment of Hb within a PC film.
- Immobilization of the PC film onto a PG electrode surface.
- Electrochemical characterization using cyclic voltammetry and amperometry.
Main Results:
- Hb exhibited direct electrochemistry and enzyme-like activity for NO reduction.
- A linear relationship was observed between peak current and NO concentration (1.0 x 10(-7)-3.0 x 10(-4) mol/L).
- A low detection limit of 1.0 x 10(-7) mol/L was achieved.
Conclusions:
- The developed Hb/PC/PG biosensor is effective for reagentless NO detection.
- The biosensor demonstrates good stability, selectivity, and ease of construction.
- This biosensor holds promise for the rapid determination of trace amounts of NO.