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A novel two-enzyme amperometric electrode for lactose determination
Handan Gülce1, Ahmet Gülce, Attila Yildiz
1Department of Chemistry, Suleyman Demirel University, Isparta, Turkey.
Abstract:
Coimmobilization of beta-galactosidase and glucose oxidase in a redox polymer, polyvinylferrocenium perchlorate (PVF+ ClO4-), led to the development of an enzyme electrode for the determination of lactose. The amperometric response of the electrode was measured at +0.70 V vs. SCE, which was due to the electrooxidation of enzymatically produced H2O2. The effects of the substrate and buffer concentrations as well as the pH on the electrode response were elucidated.