Related Experiment Video
Updated: Jul 29, 2026

12:18
An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
Summary
Serotyping of 607 Pasteurella multocida strains from poultry in Israel revealed serotypes 1 and 3 are most common. Serotype 1 was predominant, highlighting its importance in poultry health.
Area of Science:
- Veterinary Microbiology
- Avian Pathology
- Bacterial Serotyping
Background:
- Pasteurella multocida is a significant avian pathogen.
- Poultry farming in Israel involves turkeys, geese, and chickens.
- Effective disease control relies on accurate bacterial identification and characterization.
Purpose of the Study:
- To serotype Pasteurella multocida strains isolated from Israeli poultry.
- To determine the prevalence of different serotypes in turkeys, geese, and chickens.
- To assess the antigenic diversity and distribution of P. multocida strains.
Main Methods:
- Isolation of 607 P. multocida strains from turkeys, geese, and chickens.
- Serotyping using the gel-diffusion precipitin test with heat-stable saline extracts.
- Production of diagnostic antisera in chickens using formalinized bacterins.
Main Results:
- Serotypes 1 and 3 were dominant, accounting for over 58% of isolates across all poultry types.
- Serotype 1 was the most prevalent overall.
- Turkeys exhibited the greatest antigenic diversity, with some untypable strains.
- Many strains showed wide distribution across different flocks.
Conclusions:
- Serotypes 1 and 3 are key targets for P. multocida control in Israeli poultry.
- The antigenic variability, especially in turkeys, may impact vaccine efficacy.
- Further investigation into multi-antigenic strains is warranted for vaccine development.

