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Immunoassay method for quantitative determination of nisin in solution and on polymeric films
P P Leung1, M Khadre, T H Shellhammer
1Department of Food Science and Technology, The Ohio State University, Columbus, 43210, USA.
Aims:
To develop a non-competitive direct immunoassay method for quantifying nisin in solution or adsorbed to surfaces.
Methods And Results:
The developed method differs from traditional ELISA in pre-forming a complex of the nisin antibodies and the secondary antibody conjugate before addition to the nisin-coated solid phase. The modifications may help decrease interference from the nisin degradation molecules and reduce non-specific adsorption of secondary antibody conjugate. The new method has a detection limit of 65 ng nisin and correlates well (r=0.982) with the agar diffusion bioassay.
Conclusions:
A new sensitivity method was developed to determine the amounts of nisin adsorbed to a polymeric surface.
Significance And Impact Of The Study:
The new technique can provide a reproducible and accurate method to quantitate nisin molecules in solutions and those bound on polymeric surfaces, and it is free of the limitations of the traditional agar diffusion bioassay.