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Related Experiment Videos

The electrogenic Na+/HCO3- cotransporter, NBC.

M F Romero1

  • 1Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4970, USA. mfr2@po.cwru.edu

JOP : Journal of the Pancreas
|March 5, 2002
PubMed
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Electrogenic sodium-bicarbonate cotransporter (NBC) function is vital in many mammalian tissues. Research identified a single NBC gene (NBC1) encoding isoforms crucial for bicarbonate transport and physiological pH balance.

Area of Science:

  • Physiology
  • Molecular Biology
  • Biochemistry

Background:

  • Electrogenic sodium-bicarbonate cotransporter (NBC) function is essential in mammalian tissues like the kidney, pancreas, and brain.
  • Cloning efforts revealed a single cDNA, NBC/NBC1, exhibiting all functional characteristics of an electrogenic Na(+)/HCO(3)(-) cotransporter.
  • This NBC clone belongs to the bicarbonate transporter superfamily, related to anion exchangers.

Purpose of the Study:

  • To characterize the electrogenic Na(+)/HCO(3)(-) cotransporter (NBC).
  • To identify the gene and isoforms responsible for NBC function.
  • To investigate the tissue distribution and functional properties of NBC.

Main Methods:

  • Gene cloning and characterization of the NBC/NBC1 cDNA.

Related Experiment Videos

  • Antibody-based localization of NBC isoforms in various mammalian tissues.
  • Functional assays in Xenopus oocytes to determine ion selectivity and stoichiometry.

    • Main Results:

    • A single cDNA, NBC/NBC1, was identified, encoding the electrogenic Na(+)/HCO(3)(-) cotransporter.
    • Two N-terminal and one C-terminal isoforms were identified, all derived from the same gene with seemingly identical functions.
    • NBC isoforms were localized in numerous tissues, including kidney, pancreas, brain, and others.
    • Functional studies confirmed NBC's selectivity for HCO(3)(-) and Na(+), with a stoichiometry of 1 Na(+) : 2 HCO(3)(-) in Xenopus oocytes.

    Conclusions:

    • The NBC/NBC1 gene encodes the electrogenic Na(+)/HCO(3)(-) cotransporter, crucial for physiological pH regulation.
    • Multiple NBC isoforms exist, originating from a single gene, and are widely distributed across mammalian tissues.
    • The identified stoichiometry suggests potential interactions with accessory proteins for full functional regulation.