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Protein identification by in-gel digestion and mass spectrometric analysis
Alastair Aitken1, Michele Learmonth
1Department of Biomedical and Clinical Laboratory Sciences, University of Edinburgh. aitken@srv4.med.ed.ac.uk
Molecular Biotechnology
|March 6, 2002
Abstract:
This protocol details a method for the identification of proteins that have been separated by gel electrophoresis. In-gel digestion of the protein bands with trypsin followed by quadrupole ion-trap or other triple quadrupole mass spectrometry techniques is described. The proteins can be identified by database searching of the mass fingerprint of the intact peptides and of the characteristic fragment masses produced by tandem mass spectrometry.