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Finding your knockout: reverse genetics techniques for plants
Sean T May1, Deborah Clements, Malcolm J Bennett
1Nottingham Arabidopsis Stock Centre, School of Biosciences, Plant Science Division, University of Nottingham, UK. Sean_May@nasc.nott.ac.uk
Molecular Biotechnology
|March 6, 2002
Abstract:
The process of finding a mutant plant for your gene of interest has recently become far more straightforward and painless than has ever been possible before. This has come about through the production of large-scale insertional mutagenesis populations. These can now be readily screened in bulk for insertional mutants through a mixture of molecular (PCR/hybridization) and bioinformation techniques. Here we describe a step-by-step guide to the molecular protocols and a description of the bioinformatics approach.