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[The effects of rhGH on macrophages in scalded mice]
1Department of Burns, Changhai Hospital, The Second Military Medical University, Shanghai 200433 P.R. China.
Objective:
To investigate the effects of rhGH and IGF-1 on the CD14 mRNA expression and cytokine secretion of peritoneal macrophages (Mphi) in scalded rats.
Methods:
Reverse-transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosordent assay (ELISA) were employed to observe the postburn changes of CD14 expression and cytokines secretion of the Mphi treated in vivo and in vitro by rhGH and IGF-1.
Results:
It was demonstrated that in vivo postburn application of rhGH and IGF-1 could enhance the transcription of CD14 mRNA and the secretion of cytokines from the peritoneal Mphi in mice. But it seemed that there was no additive effect between the two factors. Additionally, rhGH (at 40 ng/ml) and IGF-1 (at all tested concentrations) could activate the secretion of TNF and IL-6 from cultured mice Mphi in vitro.
Conclusion:
Postburn application of rhGH might activate Mphi by IGF-1 induction, enhancing Mphi CD14 expression, which promoted more secretion of cytokines, and promote the enhancement of immune function.
Insights
Recombinant human growth hormone (rhGH) and insulin-like growth factor 1 (IGF-1) enhance macrophage CD14 mRNA expression and cytokine secretion in scalded rats, boosting immune function.
Area of Science:
- Immunology
- Endocrinology
- Burn Research
Background:
- Severe burns trigger significant inflammatory responses.
- Peritoneal macrophages (Mphi) play a crucial role in the immune response to injury.
- CD14 is a key receptor on macrophages involved in immune activation.
Purpose of the Study:
- To investigate the effects of recombinant human growth hormone (rhGH) and insulin-like growth factor 1 (IGF-1) on peritoneal macrophage (Mphi) CD14 mRNA expression and cytokine secretion in scalded rats.
- To elucidate the role of rhGH and IGF-1 in modulating the immune response post-burn.
Main Methods:
- Utilized reverse-transcription-polymerase chain reaction (RT-PCR) to quantify CD14 mRNA expression.
- Employed enzyme-linked immunosorbent assay (ELISA) to measure cytokine secretion.
- Studied Mphi responses both in vivo and in vitro following rhGH and IGF-1 treatment in scalded rat models.
Main Results:
- In vivo administration of rhGH and IGF-1 post-burn enhanced CD14 mRNA transcription and cytokine secretion from peritoneal Mphi.
- No additive effect was observed when rhGH and IGF-1 were administered together.
- In vitro, rhGH (40 ng/ml) and IGF-1 (all tested concentrations) stimulated the secretion of tumor necrosis factor (TNF) and interleukin-6 (IL-6) from cultured Mphi.
Conclusions:
- Post-burn rhGH application may activate Mphi through IGF-1 induction.
- This activation enhances Mphi CD14 expression, leading to increased cytokine secretion.
- The findings suggest rhGH and IGF-1 promote enhanced immune function following burn injury.